Generator

Part:BBa_I766677:Design

Designed by: Lauren Jann   Group: iGEM07_UCSF   (2007-08-16)


YopH-RR under strong constitutive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2703
    Illegal PstI site found at 1871
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1871
    Illegal NotI site found at 3851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3834
    Illegal XhoI site found at 1511
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2703
    Illegal PstI site found at 1871
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2703
    Illegal PstI site found at 1871
    Illegal NgoMIV site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874
    Illegal BsaI site found at 3208


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.


Source

composite part

References